Veröffentlichungen von

Veröffentlichungen

Prof. Dr.-Ing. Johannes Bongaerts

Zeitschriftenartikel

Novel recombinant aminoacylase from Paraburkholderia monticola capable of N-acyl-amino acid synthesis

Haeger, Gerrit ; Jolmes, Tristan ; Oyen, Sven ; Jaeger, Karl-Erich ; Bongaerts, Johannes ; Schörken, Ulrich ; Siegert, Petra (2024)
Applied Microbiology and Biotechnology. Berlin : Springer. (2024), H. 108. - 14 Seiten
1432-0614

Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis

Haeger, Gerrit ; Wirges, Jessika ; Tanzmann, Nicole ; Oyen, Sven ; Jolmes, Tristan ; Jaeger, Karl-Erich ; Schörken, Ulrich ; Bongaerts, Johannes ; Siegert, Petra (2023)
Microbial Cell Factories. Springer Nature. (2023), H. 22. Seite: Article number: 77 (2023)
1475-2859

New robust subtilisins from halotolerant and halophilic Bacillaceae

Falkenberg, Fabian ; Voß, Leonie ; Bott, Michael ; Bongaerts, Johannes ; Siegert, Petra (2023)
Applied Microbiology and Biotechnology. Berlin : Springer Nature. 107 (2023). Seite: 3939 - 3954
1432-0614

Biochemical characterisation of a novel broad pH spectrum subtilisin from Fictibacillus arsenicus DSM 15822ᵀ

Falkenberg, Fabian ; Kohn, Sophie ; Bott, Michael ; Bongaerts, Johannes ; Siegert, Petra (2023)
FEBS Open Bio. Hoboken, NJ : Wiley. 13 (2023), H. 11. Seite: 2035 - 2046
2211-5463

Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans

Haeger, Gerrit ; Probst, Johanna ; Jaeger, Karl-Erich ; Bongaerts, Johannes ; Siegert, Petra (2023)
FEBS Open Bio. Hoboken, NJ : Wiley. 13 (2023), H. 12. Seite: 2224 - 2238
2211-5463

Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor

Welden, Melanie ; Severins, Robin ; Poghossian, Arshak ; Wege, Christina ; Bongaerts, Johannes ; Siegert, Petra ; Keusgen, Michael ; Schöning, Michael Josef (2022)
Chemosensors. Basel : MDPI. 10 (2022), H. 6. - Artikel 218
2227-9040

Biochemical characterization of a novel oxidatively stable, halotolerant, and high-alkaline subtilisin from Alkalihalobacillus okhensis Kh10-101T

Falkenberg, Fabian ; Rahba, Jade ; Fischer, David ; Bott, Michael ; Bongaerts, Johannes ; Siegert, Petra (2022)
FEBS Open Bio. Hoboken, NJ : Wiley. 12 (2022), H. 10. Seite: 1729 - 1746
2211-5463

Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae

Falkenberg, Fabian ; Bott, Michael ; Bongaerts, Johannes ; Siegert, Petra (2022)
Frontiers in Microbiology. Lausanne : Frontiers. 2022 (2022), H. 13. - Artikel 13:1017978
1664-302X

A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent

Haeger, Gerrit ; Bongaerts, Johannes ; Siegert, Petra (2022)
Analytical Biochemistry. Amsterdam : Elsevier. (2022), H. 624. - Artikel 114819
1096-0309

Capacitive field‐effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths

Jablonski, Melanie ; Münstermann, Felix ; Nork, Jasmina ; Molinnus, Denise ; Muschallik, Lukas ; Bongaerts, Johannes ; Wagner, Torsten ; Keusgen, Michael ; Siegert, Petra ; Schöning, Michael Josef (2021)
physica status solidi (a) applications and materials science. Weinheim : Wiley-VCH. 218 (2021), H. 13. - 7 Seiten
1862-6319

Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase

Muschallik, Lukas ; Kipp, Carina Ronja ; Recker, Inga ; Bongaerts, Johannes ; Pohl, Martina ; Gelissen, Melanie ; Schöning, Michael Josef ; Selmer, Thorsten ; Siegert, Petra (2020)
Journal of Biotechnology. Amsterdam : Elsevier. 202 (2020), H. Vol. 324. Seite: 61 - 70
2590-1559

Synthesis of α-hydroxy ketones and vicinal (R, R)-diols by Bacillus clausii DSM 8716ᵀ butanediol dehydrogenase

Muschallik, Lukas ; Molinnus, Denise ; Jablonski, Melanie ; Kipp, Carina Ronja ; Bongaerts, Johannes ; Pohl, Martina ; Wagner, Torsten ; Schöning, Michael Josef ; Selmer, Thorsten ; Siegert, Petra (2020)
RSC Advances. Cambridge : Royal Society of Chemistry (RSC). 10 (2020). Seite: 12206 - 12216
2046-2069

Development and characterization of a field-effect biosensor for the detection of acetoin

Molinnus, Denise ; Muschallik, Lukas ; Gonzalez, Laura Osorio ; Bongaerts, Johannes ; Wagner, Torsten ; Selmer, Thorsten ; Siegert, Petra ; Keusgen, Michael ; Schöning, Michael Josef (2018)
Biosensors and Bioelectronics. Amsterdam : Elsevier. 115 (2018). Seite: 1 - 6

Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium

Müller, Janina ; Beckers, Mario ; Mußmann, Nina ; Bongaerts, Johannes ; Büchs, Jochen (2018)
Microbial Cell Factories. BioMed Central. 17 (2018), H. 1. - 17Seite: Article No. 106
1475-2859

(R,R)-Butane-2,3-diol Dehydrogenase from Bacillus clausii DSM 8716T: Cloning and Expression of the bdhA-Gene, and Initial Characterization of Enzyme

Muschallik, Lukas ; Molinnus, Denise ; Bongaerts, Johannes ; Pohl, Martina ; Wagner, Torsten ; Schöning, Michael Josef ; Siegert, Petra ; Selmer, Thorsten (2017)
Journal of Biotechnology. Amsterdam : Elsevier. 258 (2017). Seite: 41 - 50
0168-1656

High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium

Voigt, Birgit ; Albrecht, Dirk ; Sievers, Susanne ; Becher, Dörte ; Bongaerts, Johannes ; Evers, Stefan ; Schweder, Thomas ; Maurer, Karl-Heinz ; Hecker, Michael (2015)
Proteomics. Weinheim : Wiley. 15 (2015), H. 15. Seite: 2629 - 2633
1615-9861

Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer

Küppers, Tobias ; Steffen, Victoria ; Hellmuth, Hendrik ; O'Connell, Timothy ; Bongaerts, Johannes ; Maurer, Karl-Heinz ; Wiechert, Wolfgang (2014)
Microbial cell factories. London : BioMed Central. 13 (2014). - 11 SeitenSeite: Article No. 46
1475-2859 (E-Journal)

Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach

Handtke, Stefan ; Volland, Sonja ; Methling, Karen ; Albrecht, Dirk ; Becher, Dörte ; Nehls, Jenny ; Bongaerts, Johannes ; Maurer, Karl-Heinz ; Lalk, Michael ; Liesegang, Heiko ; Voigt, Birgit ; Daniel, Rolf ; Hecker, Michael (2014)
Journal of Biotechnology. Amsterdam : Elsevier. (2014), H. 192(A). Seite: 204 - 214
1873-4863 (E-Journal) , 0168-1656 (Print)

Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges

Schroeter, Rebecca ; Hoffmann, Tamara ; Voigt, Birgit ; Meyer, Hanna ; Bleisteiner, Monika ; Muntel, Jan ; Jürgen, Britta ; Albrecht, Dirk ; Becher, Dörte ; Lalk, Michael ; Evers, Stefan ; Bongaerts, Johannes ; Maurer, Karl-Heinz ; Putzer, Harald ; Hecker, Michael ; Schweder, Thomas ; Bremer, Erhard (2014)
PLoS ONE. San Francisco : PLOS. 8 (2014), H. 11. - e80956
1932-6203

Bacillus pumilus reveals a remarkably high resistance to hydrogen peroxide provoked oxidative stress

Handtke, Stefan ; Schroeter, Rebecca ; Jürgen, Britta ; Methling, Karen ; Schlüter, Rabea ; Albrecht, Dirk ; Hijum, Sacha A. F. T. van ; Bongaerts, Johannes ; Maurer, Karl-Heinz ; Lalk, Michael ; Schweder, Thomas ; Hecker, Michael ; Voigt, Birgit (2014)
PLOS one. San Francisco : PLOS. 9 (2014), H. 1. - e85625
1932-6203

Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol–xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum

Scheele, Sandra ; Oertel, Dan ; Bongaerts, Johannes ; Evers, Stefan ; Hellmuth, Hendrik ; Maurer, Karl-Heinz ; Bott, Michael ; Freudl, Roland (2013)
Microbial biotechnology. Oxford : Wiley-Blackwell. (2013). Seite: 202 - 206
1751-7915

Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations

Wilming, Anja ; Begemann, Jens ; Kuhne, Stefan ; Regestein, Lars ; Bongaerts, Johannes ; Evers, Stefan ; Maurer, Karl-Heinz ; Büchs, Jochen (2013)
Biochemical engineering journal. Amsterdam : Elsevier. Vol. 73 (2013). Seite: 29 - 37
1873-295X (E-Journal) , 1369-703X (Print)

The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon

Voigt, Birgit ; Schroeter, Rebecca ; Jürgen, Britta ; Albrecht, Dirk ; Evers, Stefan ; Bongaerts, Johannes ; Maurer, Karl-Heinz ; Schweder, Thomas ; Hecker, Michael (2013)
Proteomics. Weinheim : Wiley. Vol. 13 (2013), H. Iss. 14. Seite: 2140 - 2146
1615-9861 (E-Journal) , 1615-9853 (Print)

Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis

Rachinger, Michael ; Bauch, Melanie ; Strittmatter, Axel ; Bongaerts, Johannes ; Evers, Stefan ; Maurer, Karl-Heinz ; Daniel, Rolf ; Liebl, Wolfgang ; Liesegang, Heiko ; Ehrenreich, Armin (2013)
Journal of biotechnology. Amsterdam : Elsevier. Vol. 164 (2013), H. Iss. 4. Seite: 365 - 369
1873-4863 (E-Journal) , 0168-1656 (Print)

RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation

Wiegand, Sandra ; Dietrich, Sascha ; Hertel, Robert ; Bongaerts, Johannes ; Evers, Stefan ; Volland, Sonja ; Daniel, Rolf ; Liesegang, Heiko (2013)
BMC genomics. London : BioMed Central. Vol. 14 (2013). Seite: 667
1471-2164

Fermentation stage-dependent adaptations of Bacillus licheniformis during enzyme production

Wiegand, Sandra ; Voigt, Birgit ; Albrecht, Dirk ; Bongaerts, Johannes ; Evers, Stefan ; Hecker, Michael ; Daniel, Rolf ; Liesegang, Heiko (2013)
Microbial Cell Factories. London : Biomed Central. 12 (2013). Seite: 120
1475-2859

Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production

Borgmeier, Claudia ; Bongaerts, Johannes ; Meinhardt, Friedhelm (2012)
Journal of biotechnology. Amsterdam : Elsevier. 159 (2012), H. 1-2. Seite: 12 - 20
1873-4863 (E-Journal) , 0168-1656 (Print)

Enzymatic deglycation of Amadori products in bacteria

Deppe, Veronika Maria ; Bongaerts, Johannes ; O'Connell, Timothy ; Maurer, Karl-Heinz ; Meinhardt, Friedhelm (2011)
Applied microbiology and biotechnology. Berlin : Springer. Vol. 90 (2011), H. Iss. 2. Seite: 399 - 406
1432-0614 (E-Journal) , 0171-1741 (Print) , 0175-7598 (Print) , 0340-2118 (Print)

Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR

Deppe, Veronika Maria ; Klatte, Stephanie ; Bongaerts, Johannes ; Maurer, Karl-Heinz ; O'Connell, Timothy ; Meinhardt, Friedhelm (2011)
Applied and environmental microbiology. New York : American Society of Mechanical Engineers (ASME). Vol. 77 (2011), H. No. 9. Seite: 2839 - 2846
1098-5336 (E-Journal) , 0003-6919 (Print) , 0099-2240 (Print)

Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis

Bongaerts, Johannes ; Esser, Simon ; Lorbach, Volker ; Al-Momani, Lóay ; Müller, Michael A. ; Franke, Dirk ; Grondal, Christoph ; Kurutsch, Anja ; Bujnicki, Robert ; Takors, Ralf ; Raeven, Leon ; Wubbolts, Marcel ; Bovenberg, Roel ; Nieger, Martin ; Schürmann, Melanie ; Trachtmann, Natalie ; Kozak, Stefan ; Sprenger, Georg A. ; Müller, Michael (2011)
Angewandte Chemie International Edition. Weinheim : Wiley. Vol. 50 (2011), H. Iss. 34. Seite: 7781 - 7786
1521-3773 (E-Journal) , 0570-0833 (Print) , 1433-7851 (Print)

Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides

Degering, Christian ; Eggert, Thorsten ; Puls, Michael ; Bongaerts, Johannes ; Evers, Stefan ; Maurer, Karl-Heinz ; Jaeger, Karl-Erich (2010)
Applied and environmental microbiology. Washington, DC : American Society for Microbiology. 76 (2010), H. 19. Seite: 6370 - 6378
1098-5336 (E-Journal) , 0003-6919 (Print) , 0099-2240 (Print)

Sekretion einer Kofaktor-haltigen Oxidase durch Corynebacterium glutamicum

Scheele, S. ; Bongaerts, Johannes ; Maurer, K.-H. ; Freudl, R. (2009)
Chemie - Ingenieur - Technik (CIT). . Vol. 81 (2009), H. Iss. 8. Seite: 1309
1522-2640 (E-Journal) , 0009-286X (Print)

The global gene expression response of Escherichia coli to L-phenylalanine

Polen, T. ; Krämer, Marco ; Bongaerts, Johannes ; Wubbolts, Marcel ; Wendisch, V. F. (2005)
Journal of biotechnology. . Vol. 115 (2005), H. Iss. 3. Seite: 221 - 237
1873-4863 (E-Journal) , 0168-1656 (Print)

Metabolic engineering for microbial production of shikimic acid

Krämer, Marco ; Bongaerts, Johannes ; Bovenberg, Roel ; Kremer, Susanne ; Müller, Ulrike ; Orf, Sonja ; Wubbolts, Marcel ; Raeven, Leon (2003)
Metabolic engineering. . Vol. 5 (2003), H. Iss. 4. Seite: 277 - 283
1096-7184 (E-Journal) , 1096-7176 (Print)

Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli

Schmitz, M. ; Hirsch, E. ; Bongaerts, Johannes ; Takors, Ralf (2002)
Biotechnology progress. . Vol. 18 (2002), H. Iss. 5. Seite: 935 - 941
1520-6033 (E-Journal) , 8756-7938 (Print)

Metabolic engineering to produce fine chemicals in Escherichia coli

Bongaerts, Johannes ; Bovenberg, Roel ; Krämer, Marco ; Müller, Ulrike ; Raeven, Leon ; Wubbolts, Marcel (2002)
Chemie - Ingenieur - Technik (CIT). . Vol. 74 (2002), H. Iss. 5. Seite: 694
1522-2640 (E-Journal) , 0009-286X (Print)

Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains

Gerigk, M. ; Bujnicki, R. ; Ganpo-Nkwenkwa, E. ; Bongaerts, Johannes ; Sprenger, G. ; Takors, Ralf (2002)
Biotechnology and bioengineering. . Vol. 80 (2002), H. Iss. 7. Seite: 746 - 754
1097-0290 (E-Journal) , 0006-3592 (Print)

Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction

Gerigk, M. ; Maaß, D. ; Kreutzer, A. ; Sprenger, G. ; Bongaerts, Johannes ; Wubbolts, Marcel ; Takors, Ralf (2002)
Bioprocess and biosystems engineering. . Vol. 25 (2002), H. Iss. 1. Seite: 43 - 52
1432-0797 (E-Journal) , 1615-7605 (E-Journal) , 0178-515X (Print) , 1615-7591 (Print)

Metabolic engineering to produce fine chemicals in Escherichia coli

Müller, Ulrike ; Bongaerts, Johannes ; Bovenberg, Roel ; Jossek, Ralf ; Krämer, Marco ; Linnemann, J. ; Müschen, S. ; Ritterbecks, S. ; Sprenger, G. ; Wubbolts, Marcel (2001)
Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent. . 66 (3a) (2001). Seite: 215 - 217
0035-533x

Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli

Jossek, Ralf ; Bongaerts, Johannes ; Sprenger, Georg A. (2001)
FEMS microbiology letters. . Vol. 202 (2001), H. Iss. 1. Seite: 145 - 148
1574-6968

Metabolic engineering for microbial production of aromatic amino acids and derived compounds

Bongaerts, Johannes ; Krämer, Marco ; Müller, Ulrike ; Raeven, Leon ; Wubbolts, Marcel (2001)
Metabolic engineering. . Vol. 3 (2001), H. Iss. 4. Seite: 289 - 300
1096-7184 (E-Journal) , 1096-7176 (Print)

Fermentative Herstellung von L-Phenylalanin im Fed-Batch Verfahren mit E. coli unter Einbindung eines integrierten Aufarbeitungsverfahrens

Gerigk, M. ; Maaß, D. ; Takors, Ralf ; Kreutzer, A. ; Wandrey, Christian ; Bongaerts, Johannes ; Wubbolts, Marcel (2000)
Chemie-Ingenieur-Technik (CIT). . Vol. 72 (2000), H. Iss. 9. Seite: 926 - 927
1522-2640 (E-Journal) , 0009-286X (Print)

Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance

Wackwitz, B. ; Bongaerts, Johannes ; Goodman, S. D. ; Unden, Gottfried (1999)
Molecular and general genetics : MGG. . Vol. 262 (1999), H. Iss. 4 - 5. Seite: 876 - 883
1617-4623 (E-Journal) , 1617-4615 (Print)

Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system

Zientz, Evelyn ; Bongaerts, Johannes ; Unden, Gottfried (1998)
Journal of bacteriology. . Vol. 180 (1998), H. No. 20. Seite: 5421 - 5425
1098-5530 (E-Journal) , 0021-9193 (Print)

Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications

Tran, Quang Hon ; Bongaerts, Johannes ; Vlad, Dorina ; Unden, Gottfried (1997)
European journal of biochemistry. . Vol. 244 (1997), H. Iss. 1. Seite: 155 - 160
0014-2956

Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors

Unden, Gottfried ; Bongaerts, Johannes (1997)
Biochimica et biophysica acta (BBA) - Bioenergetics. . Vol. 1320 (1997), H. Iss. 3. Seite: 217 - 234
1879-2650 (E-Journal) , 0005-2728 (Print)

Transcriptional regulation of the proton translocating NADH

Bongaerts, Johannes ; Zoschke, Sascha ; Weidner, Uwe ; Linden, Gottfried (1995)
Molecular microbiology. . Vol. 16 (1995), H. Iss. 3. Seite: 521 - 534
1365-2958 (E-Journal) , 0950-382x (Print)

O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria

Unden, G. ; Becker, S. ; Bongaerts, Johannes ; Holighaus, G. ; Schirawski, J. ; Six, S. (1995)
Archives of microbiology. . Vol. 164 (1995), H. Iss. 2. Seite: 81 - 90
1432-072X (E-Journal) , 0003-9276 (Print) , 0302-8933 (Print)

A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport

Wissenbach, U. ; Six, S. ; Bongaerts, Johannes ; Ternes, D. ; Steinwachs, S. ; Unden, G. (1995)
Molecular microbiology. . Vol. 17 (1995), H. Iss. 4. Seite: 675 - 686
1365-2958 (E-Journal) , 0950-382x (Print)

Oxygen regulated gene expression in facultatively anaerobic bacteria

Unden, G. ; Becker, S. ; Bongaerts, Johannes ; Schirawski, J. ; Six, S. (1994)
Antonie van Leeuwenhoek. . Vol. 66 (1994), H. Iss. 1-3. Seite: 3 - 22
0003-6072 (Print) , 1572-9699 (online)

Patente

Wasch- oder Reinigungsmittel mit gesteigerter Waschkraft [Offenlegungsschrift]
Method from improving the cleaning action of a detergent of cleaning agent [US Patentanmeldung]

O'Connell, Timothy ; Siegert, Petra ; Evers, Stefan ; Bongaerts, Johannes ; Weber, Thomas ; Maurer, Karl-Heinz ; Bessler, Cornelius (2010)
München : Deutsches PatentamtSeite: 1 - 34

Weitere Publikationen

Abschlussbericht Teil II: Eingehende Darstellung Neue biobasierte Lipopeptide aus nachhaltiger Produktion (LipoPep)

Haeger, Gerrit ; Bongaerts, Johannes ; Siegert, Petra (2023)
- 17Seiten
Förderkennzeichen: 13FH256PA6 Titel: FHprofUnt 2016: Neue biobasierte Lipopeptide aus nachhaltiger Produktion Laufzeit: 01.02.2019 – 31.10.2022

Abschlussbericht zum Projekt zur Überwachung biotechnologischer Prozesse mittels Diacetyl-/Acetoin-Biosensor und Evaluierung von Acetoin-Reduktasen zur Verwendung in Biotransformationen

Siegert, Petra ; Bongaerts, Johannes ; Wagner, Torsten ; Schöning, Michael Josef ; Selmer, Thorsten (2022)
Aachen : - 16 Seiten
Laufzeit: 01.01.2016 – 31.12.2019 (verlängert bis 31.12.2020) Förderkennzeichen: 322-8.03.04.02-FH-Struktur 2016/02 Gefördert durch: Ministerium für Innovation, Wissenschaft und Forschung des Landes Nordrhein-Westfalen